How Much Bacteriostatic Water to Mix with 5mg of Semaglutide
Semaglutide has gained immense popularity as a medication for type 2 diabetes and weight loss under brand names like Ozempic and Wegovy. However, when using compounded semaglutide in powdered form, it’s essential to reconstitute it properly with bacteriostatic water for safe and effective use.
If you’re wondering, “How much bacteriostatic water should I mix with 5mg of semaglutide?”—you’re in the right place. This guide breaks it all down in simple, easy-to-understand terms.
Contents
What Is Semaglutide Powder?
Semaglutide powder is an active pharmaceutical ingredient (API) that must be reconstituted—meaning it must be mixed with bacteriostatic water—before it can be used via subcutaneous injection.
Reconstitution ensures the peptide is properly diluted and safe for use.
What Is Bacteriostatic Water?
Bacteriostatic water is sterile water that contains 0.9% benzyl alcohol, which acts as a preservative. It prevents bacteria from growing in the solution, making it safer for multi-dose use over time.
How Much Bacteriostatic Water to Mix with 5mg of Semaglutide?
The amount of bacteriostatic water you mix with 5mg of semaglutide depends on your desired concentration per unit and dose per injection.
Here are 3 popular dilution options to consider:
Option 1: 5mg Semaglutide + 1 mL Bacteriostatic Water
- Concentration: 5mg/mL
- Example dose: If taking 0.25mg/week, you inject 0.05 mL (or 5 units on an insulin syringe).
Option 2: 5mg Semaglutide + 2 mL Bacteriostatic Water
- Concentration: 2.5mg/mL
- Example dose: For 0.25mg/week, inject 0.1 mL (or 10 units).
Option 3: 5mg Semaglutide + 5 mL Bacteriostatic Water
- Concentration: 1mg/mL
- Example dose: For 0.25mg/week, inject 0.25 mL (or 25 units).
💡 Pro Tip: Many users prefer the 1mg/mL concentration for easier and more precise dosing using an insulin syringe marked in units (1 unit = 0.01 mL).
Safe Handling and Storage
- Use a sterile vial for mixing
- Always mix in a clean environment
- Store the mixed solution in the refrigerator (36°F to 46°F / 2°C to 8°C)
- Discard the solution after 28–30 days or as directed
- Never use cloudy or discolored solution
Important Disclaimer
This article is for educational purposes only. Always consult with a licensed healthcare provider before using any compounded medications or performing injections at home. Dosing may vary based on individual needs and health conditions.
Summary Table: Mixing Semaglutide with Bacteriostatic Water
Amount of Bacteriostatic Water | Final Concentration | 0.25mg Dose Volume |
---|---|---|
1 mL | 5mg/mL | 0.05 mL (5 units) |
2 mL | 2.5mg/mL | 0.1 mL (10 units) |
5 mL | 1mg/mL | 0.25 mL (25 units) |
Final Thoughts
Knowing how much bacteriostatic water to mix with 5mg of semaglutide is crucial for accurate dosing and safety. Whether you’re just starting or adjusting your injection regimen, choosing the right dilution and understanding how it affects your dosage can make your journey smoother and more effective.
Always follow medical advice, use sterile techniques, and store your medication properly to get the most from your semaglutide treatment.

Steve George is Blogger, a marketer and content writer. He has B.A. in Economics from the University of Washington. Read more about Mzuri Mag.